A Kinesin-Related Protein, Krp180, Positions Prometaphase Spindle Poles during Early Sea Urchin Embryonic Cell Division
نویسندگان
چکیده
We have investigated the intracellular roles of an Xklp2-related kinesin motor, KRP(180), in positioning spindle poles during early sea urchin embryonic cell division using quantitative, real-time analysis. Immunolocalization reveals that KRP(180) concentrates on microtubules in the central spindle, but is absent from centrosomes. Microinjection of inhibitory antibodies and dominant negative constructs suggest that KRP(180) is not required for the initial separation of spindle poles, but instead functions to transiently position spindle poles specifically during prometaphase.
منابع مشابه
Prometaphase Spindle Poles during Early Sea Urchin Embryonic Cell Division
We have investigated the intracellular roles of an Xklp2-related kinesin motor, KRP 180 , in positioning spindle poles during early sea urchin embryonic cell division using quantitative, real-time analysis. Immunolocalization reveals that KRP 180 concentrates on microtubules in the central spindle, but is absent from centrosomes. Microinjection of inhibitory antibodies and dominant negative con...
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ورودعنوان ژورنال:
- The Journal of Cell Biology
دوره 150 شماره
صفحات -
تاریخ انتشار 2000